Performance of HPLC/MS microchips in isocratic and gradient elution modes
نویسندگان
چکیده
منابع مشابه
Isocratic and gradient elution chromatography: a comparison in terms of speed, retention reproducibility and quantitation.
Chromatographers are cautioned to avoid gradient elution when isocratic elution will do. In this work, we compared the analytical properties of gradient and isocratic separations of a sample which can be done quite readily under isocratic conditions. We found that gradient elution gave a shorter overall analysis with similar resolution of the critical pair compared to isocratic elution without ...
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There is great interest in using microfluidic channels packed with a stationary phase for chemical separations of complex mixtures. A key advantage of such techniques is the use of electroosmotic flow (EOF), controlled simply by applying electrical potentials between reservoirs. A disadvantage for this technique, however, is a lack of compatibility with gradient elution separations. This limita...
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ژورنال
عنوان ژورنال: Journal of Mass Spectrometry
سال: 2010
ISSN: 1076-5174,1096-9888
DOI: 10.1002/jms.1719